Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels
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منابع مشابه
Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels
BACKGROUND Single chain Fvs (scFvs) are widely applied in research, diagnostics and therapeutic settings. Display and selection from combinatorial libraries is the main route to their discovery and many factors influence the success of this process. They exhibit low thermodynamic stability, resulting in low levels of premature cytosolic folding or aggregation which facilitates sec YEG-mediated ...
متن کاملISOLATION BY PHAGE DISPLAY OF SINGLE-CHAIN Fv ANTIBODIES AGAINST Escerichia coli K99 FIMBRIAE
Single-chain antibodies (scFv) are small, recombinant proteins in which variable domains from immunoglobulin heavy and light chains are physically linked with a flexible peptide. They are monovalent and lack the heavy chain constant domins for activation of complement and binding to cellular receptors. However scFvs can possess biological activity by binding to and inhibiting the action of thei...
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BACKGROUND Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from other species can, however, also be used. Because of the way in which gene conversion introduces diversity, the naïve antibody repertoire of the chicken can easily be accessed usi...
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Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplas...
متن کاملMammalian expression and secretion of functional single-chain Fv molecules.
Single-chain Fv (sFv) proteins are genetically engineered molecules that consist of the two variable domains of an antibody connected by a polypeptide linker; they contain the antigen binding function of the parental protein in a single 30-kDa polypeptide chain. sFvs are usually produced in bacteria where they are insoluble and therefore require extensive refolding in vitro. In this report we f...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2008
ISSN: 1472-6750
DOI: 10.1186/1472-6750-8-97